FSTL1 Kit ELISA

N° du produit ABIN6719929

N° du produit (Fournisseur): koa0595

Kit ELISA FSTL1 Humain, Colorimetric test pour la quantification de Humain FSTL1.

Aperçu rapide pour FSTL1 Kit ELISA (ABIN6719929)

Antigène: FSTL1 (Follistatin-Like 1 (FSTL1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit FSTL1 Kit ELISA

Seuil minimal de détection: 312 pg/mL

N° du produit (Fournisseur): koa0595

Fournisseur: Rockland

Fonction: Human FSTL1 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: M1-I308

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human FSTL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. It is recommended that each human FSTL1 standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 4.5%, 7.4%, 4%
Inter-Assay Precision (CV%): 7.8%, 7.8%, 4.1%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: FSTL1 (Follistatin-Like 1 (FSTL1))

Autre désignation: FSTL1

Sujet:

Background: Follistatin-related protein 1, also called FSTL1 or FRP is a protein that in humans is encoded by the FSTL1 gene. FSTL1 gene is mapped to chromosome 3q13.33 based on an alignment of FSTL1 sequence with the genomic sequence (GRCh37). This gene encodes a protein with similarity to follistatin, an activin-binding protein. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. This gene product is thought to be an autoantigen associated with rheumatoid arthritis. This gene may modulate the action of some growth factors on cell proliferation and differentiation. It was suggested that fibroblast-secreted FSTL1 acts on macrophages to increase TNF and IL1B production and IL6 activity.

Gene Full Name: follistatin like 1

ID gène: 11167

NCBI Accession: NP_009016

UniProt: Q12841