alpha Fetoprotein Kit ELISA
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- Antigène Voir toutes alpha Fetoprotein (AFP) Kits ELISA
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 78 pg/mL - 5000 pg/mL
- Seuil minimal de détection
- 78 pg/mL
- Application
- ELISA
- Fonction
- Sandwich ELISA for Quantitative Detection of Antigen
- Type d'échantillon
- Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- Attributs du produit
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Synonyms: Afp, Alpha fetoglobulin, Alpha-fetoglobulin, Alpha fetoprotein, Alpha-fetoprotein, Alpha-1-fetoprotein, FETA, HPAFP
Background: Alpha-fetoprotein (AFP, α-fetoprotein, also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown, however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.
Gene Name: AFP
Production: Natural and recombinant human AFP. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: R19-V609
- Top Product
- Discover our top product AFP Kit ELISA
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- Indications d'application
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human AFP standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. We recommend that each human AFP standard solution and each sample is measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 78pg/mL-5000pg/mL
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Antigène Voir toutes alpha Fetoprotein (AFP) Kits ELISA
- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
- Autre désignation
- AFP (AFP Produits)
- Synonymes
- FETA Kit ELISA, HPAFP Kit ELISA, alpha fetoprotein Kit ELISA, alpha-fetoprotein Kit ELISA, AFP Kit ELISA, Afp Kit ELISA
- ID gène
- 101
- NCBI Accession
- NP_001100
- UniProt
- P78325
- Pathways
- C21-Steroid Hormone Metabolic Process
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