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Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1) Kit ELISA

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain .
Rockland
N° du produit ABIN6719942
N° du produit (Fournisseur): koa0332

Aperçu rapide pour Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1) Kit ELISA (ABIN6719942)

Antigène

Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1)

Reactivité

Humain

Méthode de détection

Colorimetric

Type de méthode

Sandwich ELISA

Gamme de detection

156 pg/mL - 10000 pg/mL

Application

ELISA

Type d'échantillon

Cell Culture Supernatant, Plasma (EDTA), Serum
  • Seuil minimal de détection

    156 pg/mL

    N° du produit (Fournisseur)

    koa0332

    Fournisseur

    Rockland

    Fonction

    Human sVEGFR1 - sFLT1 Sandwich ELISA Kit for Quantitative Detection

    Marque

    AccuSignal™

    Analytical Method

    Quantitative

    Specificité

    Production: Natural and recombinant human Svegfr1. There is no detectable cross-reactivity with other relevant proteins.

    Sensibilité

    < 30 pg/mL

    Ingrédients

    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
  • Indications d'application

    ELISA_Dilution: 156pg/mL-10000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human sVEGFR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. It is recommended that each human sVEGFR1 standard solution and each sample be measured in duplicate.

    Commentaires

    Standard: Expression system for standard: sf21, Immunogen sequence: S27-H687

    Volume d'échantillon

    100 μL

    Plaque

    Pre-coated

    Restrictions

    For Research Use only
  • Stock

    4 °C,-20 °C

    Stockage commentaire

    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

    Date de péremption

    12 months
  • Antigène

    Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR-1)

    Autre désignation

    sVEGFR1/sFLT1

    Sujet

    Synonyms: FLT, FLT 1, Flt-1, FLT1, Fms like tyrosine kinase 1, Fms related tyrosine kinase 1, Fms-like tyrosine kinase 1, Tyrosine protein kinase FRT, Tyrosine protein kinase receptor FLT, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, Vascular endothelial growth factor receptor 1, Vascular endothelial growth factor/vascular permeability factor receptor, Vascular permeability factor receptor, VEGFR 1, VEGFR-1, VEGFR1, VGFR1_HUMAN

    Background: sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.

    ID gène

    2321

    NCBI Accession

    NP_001153392

    UniProt

    P17948
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