Thymic Stromal Lymphopoietin Kit ELISA

N° du produit ABIN6719962

N° du produit (Fournisseur): koa0703

Kit ELISA Thymic Stromal Lymphopoietin Souris, Colorimetric test pour la quantification de Souris Thymic Stromal Lymphopoietin.

Aperçu rapide pour Thymic Stromal Lymphopoietin Kit ELISA (ABIN6719962)

Antigène: Thymic Stromal Lymphopoietin (TSLP)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit Thymic Stromal Lymphopoietin Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

N° du produit (Fournisseur): koa0703

Fournisseur: Rockland

Fonction: Mouse TSLP Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: Y20-E140

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse TSLP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TSLP standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 5.3%, 6%, 7.8%
Inter-Assay Precision (CV%): 5.3%, 6.2%, 9%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Thymic Stromal Lymphopoietin (TSLP)

Autre désignation: TSLP

Sujet:

Background: Thymic stromal lymphopoietin, also called TSLP, is a protein belonging to the cytokine family. This gene is mapped to 5q22.1. It encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c (+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases.

Gene Full Name: thymic stromal lymphopoietin

ID gène: 53603

NCBI Accession: NP_067342

UniProt: Q9JIE6