CCL16 Kit ELISA

N° du produit ABIN6719980

N° du produit (Fournisseur): koa0645

Kit ELISA Humain CCL16, test Colorimetric pour la quantification de Humain CCL16.

Aperçu rapide pour CCL16 Kit ELISA (ABIN6719980)

Antigène: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8 pg/mL - 500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit CCL16 Kit ELISA

Seuil minimal de détection: 7.8 pg/mL

N° du produit (Fournisseur): koa0645

Fournisseur: Rockland

Fonction: Human CCL16 - HCC-4 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human CCL16. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human CCL16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CCL16 standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: Q24-Q120

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CCL16 (Chemokine (C-C Motif) Ligand 16 (CCL16))

Autre désignation: CCL16/HCC-4

Sujet:

Synonyms: C-C motif chemokine 16, CCL16, CCL16_HUMAN, Chemokine CC-4, Chemokine LEC, CKb12, HCC-4, HCC4, IL-10-inducible chemokine, IL10 inducible chemokine, ILINCK, LCC-1, LCC1, Liver CC chemokine 1 precursor, Liver expressed chemokine, Liver-expressed chemokine, LMC, Lymphocyte and monocyte chemoattractant, Monotactin 1, MTN-1, Mtn1,NCC4, New CC chemokine 4, SCYA16, SCYL4, Small inducible cytokine A16 precursor, Small inducible cytokine subfamily A (Cys Cys) member 16, Small-inducible cytokine A16

Background: Chemokine(C-C motif) ligand 16(CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine(LEC) and Monotactin-1(MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-gamma and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8.

ID gène: 6360

NCBI Accession: NP_004581

UniProt: O15467