CCL4 Kit ELISA

N° du produit ABIN6719994

Détail du produit CCL4 Kit ELISA

Antigène: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: ACT-2, ACT2, AT744.1, C-C motif chemokine 4, CC chemokine ligand 4, CCL4, CCL4_HUMAN, G-26 T-lymphocyte-secreted protein, HC21, LAG-1, LAG1, Lymphocyte activation gene 1 protein, Macrophage inflammatory protein 1-beta, MIP-1-beta, MIP-1-beta(1-69), MIP-1-beta(3-69), MIP1-B, MIP1B, MIP1B1, PAT 744, Protein H400, SCYA2, SCYA4, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2

Background: Chemokine(C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines(11, 14).

Gene Name: CCL4

Production: Natural and recombinant rat CCL4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: A24-N92

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat CCL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each rat CCL4 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CCL4 (Chemokine (C-C Motif) Ligand 4 (CCL4))

Autre désignation: CCL4 - MIP-1 beta (CCL4 Produits)

Synonymes: ACT2 Kit ELISA, AT744.1 Kit ELISA, G-26 Kit ELISA, HC21 Kit ELISA, LAG-1 Kit ELISA, LAG1 Kit ELISA, MIP-1-beta Kit ELISA, MIP1B Kit ELISA, MIP1B1 Kit ELISA, SCYA2 Kit ELISA, SCYA4 Kit ELISA, CCL4 Kit ELISA, K203 Kit ELISA, Mip1-b Kit ELISA, Scya4 Kit ELISA, MIP-1beta Kit ELISA, MIP-1BETA Kit ELISA, MIP1b Kit ELISA, SCY4A Kit ELISA, Act-2 Kit ELISA, MIP-1B Kit ELISA, Mip1b Kit ELISA, ACT-2 Kit ELISA, AT744.2 Kit ELISA, CCL4L Kit ELISA, SCYA4L Kit ELISA, SCYA4L1 Kit ELISA, SCYA4L2 Kit ELISA, C-C motif chemokine ligand 4 Kit ELISA, chemokine (C-C motif) ligand 4 Kit ELISA, chemokine (C-C motif) ligand 4-like 1 Kit ELISA, C-C motif chemokine ligand 4 like 1 Kit ELISA, CCL4 Kit ELISA, Ccl4 Kit ELISA, CCL4L1 Kit ELISA

ID gène: 116637

NCBI Accession: NP_446310

UniProt: P50230