CCL3 Kit ELISA

N° du produit ABIN6720004

N° du produit (Fournisseur): koa0258

Kit ELISA Souris CCL3, test Colorimetric pour la quantification de Souris CCL3 et a été mentionné dans 2+ publications.

Aperçu rapide pour CCL3 Kit ELISA (ABIN6720004)

Antigène: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8 pg/mL - 500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Détail du produit CCL3 Kit ELISA

Seuil minimal de détection: 7.8 pg/mL

N° du produit (Fournisseur): koa0258

Fournisseur: Rockland

Fonction: Mouse CCL3 - MIP1 alpha Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant mouse CCL3. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL mouse CCL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. We recommend that each mouse CCL3 standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence:  A24-A92

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Références pour CCL3 Kit ELISA (ABIN6720004)

Wang, Huang, Lu, Liu, Mo: "Immuno-protective vesicle-crosslinked hydrogel for allogenic transplantation." dans: Nature communications, Vol. 15, Issue 1, pp. 5176, (2024) (PubMed).

Nian, Li, Ma, Li, Li, Zhang, Ohiolei, Li, Dai, Liu, Yan, Fu, Xiao, Jia: "Understanding pathogen-host interplay by expression profiles of lncRNA and mRNA in the liver of Echinococcus multilocularis-infected mice." dans: PLoS neglected tropical diseases, Vol. 16, Issue 5, pp. e0010435, (2022) (PubMed).

Détail du antigène

Antigène: CCL3 (Chemokine (C-C Motif) Ligand 3 (CCL3))

Autre désignation: CCL3/MIP1 alpha

Sujet:

Synonyms: C C motif chemokine 3, CCL3, CCL3_HUMAN, Chemokine ligand 3, Chemokine ligand 3, G0/G1 switch regulatory protein 19 1, G0/G1 switch regulatory protein 19-1, G0S19 1, Heparin binding chemotaxis protein, LD78-alpha, Macrophage inflammatory protein 1 alpha, Macrophage inflammatory protein 1-alpha, MIP 1 alpha, MIP-1-alpha, MIP-1-alpha, MIP1A, PAT 464.1, SCYA 3, SCYA3, SIS alpha, SIS beta, SIS-beta, Small inducible cytokine A3, Tonsillar lymphocyte LD78 alpha protein

Background: Chemokine(C-C motif) ligand 3(CCL3), so known as Macrophage inflammatory protein-1alpha(MIP-1 alpha), is a protein that in humans is encoded by the CCL3 gene. It is a cytokine which belongs to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This gene is mapped to 17q12. CCL3 plays a role in inflammatory responses through binding to the receptors CCR1, CCR4 and CCR5. It has been found that the activation of CCR5 by CCL3 directly and independently activates a G-protein signaling pathway through GNAI2 and a tyrosine phosphorylation signaling pathway through JAK2.

ID gène: 20302

NCBI Accession: NP_035467

UniProt: P10855

Pathways: Cellular Response to Molecule of Bacterial Origin, Autophagy