KNG1 Kit ELISA

N° du produit ABIN6720011

Détail du produit KNG1 Kit ELISA

Antigène: KNG1 (Kininogen 1 (KNG1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Alpha-2-thiol proteinase inhibitor, BDK, BK, BK, included, bradykinin, bradykinin, included, Fitzgerald factor, Fitzgerald factor, included, Flaujeac factor, included, High molecular weight kininogen, High molecular weight kininogen, included, HMWK, HMWK, included, Ile-Ser-Bradykinin, Kallidin I, Kallidin II, kininogen 1, KNG, KNG1, KNG1_HUMAN, LMWK, included, Low molecular weight growth-promoting factor, Low molecular weight kininogen, included, Williams factor, included, Williams-Fitzgerald-Flaujeac factor

Background: Kininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor, Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor, is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins - high - molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.

Gene Name: KNG1

Production: Natural and recombinant human Kininogen-1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q19-S644

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Kininogen-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human Kininogen-1 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 0.78 ng/mL-50 ng/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: KNG1 (Kininogen 1 (KNG1))

Autre désignation: Kininogen-1 - KNG1 (KNG1 Produits)

Synonymes: BDK Kit ELISA, BK Kit ELISA, KNG Kit ELISA, Kng Kit ELISA, KNG2 Kit ELISA, fb64g01 Kit ELISA, wu:fb64g01 Kit ELISA, zgc:103569 Kit ELISA, kininogen Kit ELISA, bdk Kit ELISA, kng Kit ELISA, KNG1 Kit ELISA, IHRP Kit ELISA, KINKG Kit ELISA, KINKH Kit ELISA, Kng1 Kit ELISA, Kngk Kit ELISA, kininogen 1 Kit ELISA, kininogen 1 L homeolog Kit ELISA, inter-alpha-trypsin inhibitor heavy chain family member 4 Kit ELISA, kininogen 2-like 1 Kit ELISA, KNG1 Kit ELISA, Kng1 Kit ELISA, kng1 Kit ELISA, kng1.L Kit ELISA, ITIH4 Kit ELISA, Kng2l1 Kit ELISA

ID gène: 3827

NCBI Accession: NP_000884

UniProt: P01042

Pathways: ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process