IGFBP2 Kit ELISA

N° du produit ABIN6720032

Détail du produit IGFBP2 Kit ELISA

Antigène: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 125 pg/mL - 8000 pg/mL

Seuil minimal de détection: 125 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: BP 2, BP2, IBP 2, IBP-2, IBP2, IBP2_HUMAN, IGF binding protein 2, IGF BP53, IGF-binding protein 2, IGF-BP53, IGFBP-2, IGFBP2, IGFBP53, Insulin Like Growth Factor Binding Protein 2, Insulin like growth factor binding protein 2 36 kDa, Insulin like growth factor-binding protein 2 precursor, Insulin-like growth factor-binding protein 2

Background: Insulin-like growth factor-binding protein 2(IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment.

Gene Name: IGFBP2

Production: Natural and recombinant mouse IGFBP2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: E35-Q305

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 8000pg/mL, 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL mouse IGFBP2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IGFBP2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 125pg/mL-8000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: IGFBP2 (Insulin-Like Growth Factor Binding Protein 2, 36kDa (IGFBP2))

Autre désignation: IGFBP2 (IGFBP2 Produits)

Synonymes: IBP2 Kit ELISA, IGF-BP53 Kit ELISA, AI255832 Kit ELISA, IBP-2 Kit ELISA, Igfbp-2 Kit ELISA, mIGFBP-2 Kit ELISA, IGFBP-2 Kit ELISA, ILGFBPA Kit ELISA, pIGFBP-2 Kit ELISA, igfbp2 Kit ELISA, MGC65741 Kit ELISA, MGC76823 Kit ELISA, MGC174445 Kit ELISA, IGFBP2 Kit ELISA, ibp2 Kit ELISA, igf-bp53 Kit ELISA, igfbp2a Kit ELISA, si:ch211-2k18.3 Kit ELISA, insulin like growth factor binding protein 2 Kit ELISA, insulin-like growth factor binding protein 2 Kit ELISA, insulin-like growth factor binding protein 2a Kit ELISA, insulin-like growth factor binding protein 2b Kit ELISA, IGFBP2 Kit ELISA, Igfbp2 Kit ELISA, igfbp2a Kit ELISA, igfbp2 Kit ELISA, igfbp2b Kit ELISA

ID gène: 16008

NCBI Accession: NP_032368

UniProt: P47877

Pathways: Myometrial Relaxation and Contraction, Growth Factor Binding, Activated T Cell Proliferation