Prokineticin 1 Kit ELISA

N° du produit ABIN6720042

N° du produit (Fournisseur): koa0351

Le kit ELISA Prokineticin 1 (ABIN6720042) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Prokineticin 1.

Aperçu rapide pour Prokineticin 1 Kit ELISA (ABIN6720042)

Antigène: Prokineticin 1 (Prok1)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit Prokineticin 1 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

N° du produit (Fournisseur): koa0351

Fournisseur: Rockland

Fonction: Human EG-VEGF Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: A20-F105

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 7 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human EG-VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human EG-VEGF standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 4.7%, 7.3%, 6.5%
Inter-Assay Precision (CV%): 5.2%, 8.3%, 8%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Prokineticin 1 (Prok1)

Autre désignation: EG-VEGF

Sujet:

Background: EG-VEGF, Endocrine glandderived vascular endothelial growth factor, is a human protein encoded by by the PROKR1 gene. Its gene is mapped to chromosome 1. The 1.4-kb cDNA encodes a protein of 105 amino acids with a well defined signal sequence. The mature protein is predicted to have 86 amino acids. Northern blot analysis demonstrated expression in testis, ovary, adrenal gland, and placenta. EG-VEGF has played a vital role in inducing proliferation, migration, and fenestration in capillary endothelial cells derived from endocrine glands. The standards of this kit are recombinant human EG-VEGF (A20-F105), with molecular weight of 10.5 KDa.

Gene Full Name: prokineticin 1

ID gène: 84432

NCBI Accession: NP_115790

UniProt: P58294