IL-37 Kit ELISA

N° du produit ABIN6720052

N° du produit (Fournisseur): koa0824

Kit ELISA Humain IL-37, test Colorimetric pour la quantification de Humain IL-37.

Aperçu rapide pour IL-37 Kit ELISA (ABIN6720052)

Antigène: IL-37 (IL37) (Interleukin 37 (IL37))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit IL-37 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

N° du produit (Fournisseur): koa0824

Fournisseur: Rockland

Fonction: Human IL-37 - IL1F7 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human IL-37. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application:

ELISA_Dilution: 15.6 pg/mL-1000 pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human IL-37 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human IL-37 standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: V46-D218

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-37 (IL37) (Interleukin 37 (IL37))

Autre désignation: IL-37/IL1F7

Sujet:

Synonyms: FIL1, FIL1 zeta, FIL1(ZETA), FIL1Z, IL 1 zeta, IL 1F7, IL 1H4, IL 1RP1, IL 1X, IL 1X protein, IL-1 zeta, IL-1F7, IL-1H, IL-1H4, IL-1RP1, IL-1X, IL-37, IL1F7, IL1F7_HUMAN, IL1H4, IL1RP1, Interleukin 1 family member 7, interleukin 1 family member 7 (zeta), Interleukin 1 homolog 4, Interleukin 1 related protein, Interleukin 1 superfamily z, Interleukin 1 zeta, Interleukin 37, Interleukin-1 family member 7, Interleukin-1 homolog 4, Interleukin-1 zeta, Interleukin-1-related protein, Interleukin-23

Background: Interleukin 37(IL-37) also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. And it is mapped to 2q13. In vitro expression analysis showed that IL-37 was a secretory protein. And IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 can emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.

ID gène: 27178

NCBI Accession: NP_055254

UniProt: Q9NZH6