CD22 Kit ELISA

N° du produit ABIN6720081

N° du produit (Fournisseur): koa0777

Ce kit ELISA Souris CD22 est un kit ELISA Colorimetric conçu pour quantifier Souris CD22.

Aperçu rapide pour CD22 Kit ELISA (ABIN6720081)

Antigène: CD22 (CD22 Molecule (CD22))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit CD22 Kit ELISA

Seuil minimal de détection: 156 pg/mL

N° du produit (Fournisseur): koa0777

Fournisseur: Rockland

Fonction: Mouse CD22 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant mouse CD22. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CD22 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD22 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: S22-R702

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CD22 (CD22 Molecule (CD22))

Autre désignation: CD22

Sujet:

Synonyms: B cell receptor CD22 precursor, B lymphocyte cell adhesion molecule, B-cell receptor CD22, B-lymphocyte cell adhesion molecule, BL CAM, BL-CAM, BLCAM, CD 22, CD22, CD22 antigen, CD22 Molecule, CD22 protein, CD22_HUMAN, Lectin 2, Leu14, Lyb8, MGC130020, sialic acid binding Ig like lectin 2, Sialic acid binding immunoglobulin like lectin 2, Sialic acid-binding Ig-like lectin 2, SIGLEC 2, Siglec-2, SIGLEC2, T cell surface antigen Leu 14, T-cell surface antigen Leu-14

Background: CD22 or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. This gene is mapped to 19q13.2. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. It is a negative regulator of antigen receptor signaling whose onset of expression at the mature B cell stage may serve to raise the antigen concentration threshold required for B cell triggering. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signalling. This gene can downmodulate signaling through the IgM and IgD B-cell receptors(BCRs), but not through the IgG BCR, because the IgG cytoplasmic tail prevents CD22 phosphorylation and actually enhances IgG-BCR signaling.

ID gène: 12483

NCBI Accession: NP_001036782

UniProt: P35329