PF4 Kit ELISA

N° du produit ABIN6720101

N° du produit (Fournisseur): koa0423

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain PF4.

Aperçu rapide pour PF4 Kit ELISA (ABIN6720101)

Antigène: PF4 (Platelet Factor 4 (PF4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit PF4 Kit ELISA

Seuil minimal de détection: 156 pg/mL

N° du produit (Fournisseur): koa0423

Fournisseur: Rockland

Fonction: Human CXCL4 - PF4 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human CXCL4. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 15 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application:

ELISA_Dilution: 156pg/mL-10000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CXCL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CXCL4 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: E32-S101

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: PF4 (Platelet Factor 4 (PF4))

Autre désignation: CXCL4/PF4

Sujet:

Synonyms: C X C motif chemokine 4, C-X-C motif chemokine 4, Chemokine (C X C motif) ligand 4, Chemokine (CXC motif) ligand 4, Chemokine, CXC motif, ligand 4, CXCL 4, CXCL4, Iroplact, MGC138298, Oncostatin A, Oncostatin-A, OncostatinA, PF 4, PF-4, PF4, Pf4a, Platelet factor 4, PLF4_HUMAN, RATPF4A, SCYB 4, SCYB4, short form, Small inducible cytokine subfamily B, member 4, Small inducible cytokine subfamily member 4

Background: Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

ID gène: 5196

NCBI Accession: NP_002610

UniProt: P02776