CD31 Kit ELISA

N° du produit ABIN6720113

N° du produit (Fournisseur): koa0527

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain CD31.

Aperçu rapide pour CD31 Kit ELISA (ABIN6720113)

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit CD31 Kit ELISA

Seuil minimal de détection: 156 pg/mL

N° du produit (Fournisseur): koa0527

Fournisseur: Rockland

Fonction: Human PECAM-1 - CD31 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human PECAM-1. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human PECAM-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human PECAM-1 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: CHO, Immunogen sequence: Q28-K601

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: PECAM-1/CD31

Sujet:

Synonyms: CD31, CD31 antigen, EndoCAM, GPIIA, HEC7, Leukocyte surface protein, PECA1, PECA1_HUMAN, PECAM 1, PECAM-1, Pecam1, Platelet endothelial cell adhesion molecule, Platelet/endothelial cell adhesion molecule

Background: Human platelet/endothelial cell adhesion molecule-1(PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in the region q23-qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM-1 in regulating platelet-collagen interactions, 2 different approaches were applied using recombinant human PECAM-1-immunoglobulin chimeras and platelets derived from PECAM-1-deficient mice.3 PECAM-1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 574 amino acids(Q28-K601) with the molecular weight of 64.5KDa. After glycosylating, its molecular weight changes to 90-100KDa.

ID gène: 5175

NCBI Accession: NP_000433

UniProt: P16284

Pathways: Regulation of Actin Filament Polymerization