TPSAB1 Kit ELISA

N° du produit ABIN6720119

N° du produit (Fournisseur): koa0539

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain TPSAB1.

Aperçu rapide pour TPSAB1 Kit ELISA (ABIN6720119)

Antigène: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit TPSAB1 Kit ELISA

Seuil minimal de détection: 156 pg/mL

N° du produit (Fournisseur): koa0539

Fournisseur: Rockland

Fonction: Human Tryptase - TPSAB1 B2 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human Tryptase. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 15 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application:

ELISA_Dilution: 156pg/mL-10000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Tryptase standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Tryptase standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: M1-V275

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Autre désignation: Tryptase/TPSAB1,B2

Sujet:

Synonyms: Tryptase-1, Tryptase1, Tryptase 1, TPS1, TPS2, TPSB1, Tryptase-2, Tryptase2, Tryptase 2, Tryptase-II, TryptaseII, Tryptase II, TPSB2, Tryptase alpha/beta-1, Tryptase beta-2

Background: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles, TPSB2, which represents the beta-II and beta-III tryptase alleles, TPSG1, and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

ID gène: 7177

NCBI Accession: NP_003285

UniProt: Q15661