TPSAB1 Kit ELISA

N° du produit ABIN6720119

Détail du produit TPSAB1 Kit ELISA

Antigène: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Tryptase-1, Tryptase1, Tryptase 1, TPS1, TPS2, TPSB1, Tryptase-2, Tryptase2, Tryptase 2, Tryptase-II, TryptaseII, Tryptase II, TPSB2, Tryptase alpha/beta-1, Tryptase beta-2

Background: Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles, TPSB2, which represents the beta-II and beta-III tryptase alleles, TPSG1, and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Gene Name: Tryptase

Production: Natural and recombinant human Tryptase. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: M1-V275

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Tryptase standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Tryptase standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Other Performance Data: DOE: 2-8° C - 04/15/19 -20° C - 10/15/19

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Autre désignation: Tryptase - TPSAB1,B2 (TPSAB1 Produits)

Synonymes: TPS1 Kit ELISA, TPS2 Kit ELISA, TPSB1 Kit ELISA, PMCT7 Kit ELISA, tpsab1 Kit ELISA, tryptaseB Kit ELISA, tryptaseC Kit ELISA, MMCP-7 Kit ELISA, Mcp-7 Kit ELISA, Mcp7 Kit ELISA, Mcpt7 Kit ELISA, rMCP-7 Kit ELISA, AV011504 Kit ELISA, MMCP-6 Kit ELISA, Mcp-6 Kit ELISA, Mcp6 Kit ELISA, Mcpt6 Kit ELISA, tryptase alpha/beta 1 Kit ELISA, tryptase Kit ELISA, tryptase beta 2 (gene/pseudogene) Kit ELISA, tryptase beta 2 Kit ELISA, TPSAB1 Kit ELISA, MCT7 Kit ELISA, tpsab1 Kit ELISA, TPSB2 Kit ELISA, Tpsab1 Kit ELISA, Tpsb2 Kit ELISA

ID gène: 7177

NCBI Accession: NP_003285

UniProt: Q15661