CD36 (SR-B3) Kit ELISA

N° du produit ABIN6720127

Détail du produit

Antigène: CD36 (SR-B3)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD36 antigen, CD36 antigen (collagen type I receptor thrombospondin receptor), CD36 Molecule, CD36 Molecule (thrombospondin receptor), CHDS7, Cluster determinant 36, FAT, Fatty acid translocase, Glycoprotein IIIb, GP3B, GP4, GPIIIB, GPIV, Leukocyte differentiation antigen CD36, PAS 4 protein, PAS IV, PASIV, Platelet collagen receptor, Platelet glycoprotein 4, Platelet glycoprotein IV, SCARB3, Scavenger receptor class B member 3, Thrombospondin receptor

Background: CD36(Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV(gpIV) and glycoprotein IIIb(gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.

Gene Name: CD36

Production: Natural and recombinant mouse CD36. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: G30-K439

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse CD36 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD36 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CD36 (SR-B3)

Autre désignation: CD36 - SR-B3

ID gène: 12491

NCBI Accession: NP_001153027

UniProt: Q08857