Myeloperoxidase Kit ELISA
Aperçu rapide pour Myeloperoxidase Kit ELISA (ABIN6720134)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 156 pg/mL
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N° du produit (Fournisseur)
- koa0578
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Fournisseur
- Rockland
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Fonction
- Mouse MPO Sandwich ELISA Kit for Quantitative Detection
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Marque
- AccuSignal™
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Analytical Method
- Quantitative
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Specificité
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Production: Natural and recombinant mouse MPO. There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 10 pg/mL
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Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Indications d'application
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ELISA_Dilution: 156pg/mL-10000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse MPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse MPO standard solution and each sample be measured in duplicate.
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Commentaires
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Standard: Expression system for standard: NSO, Immunogen sequence: M16-T718
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Restrictions
- For Research Use only
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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- Myeloperoxidase (MPO)
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Autre désignation
- MPO
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Sujet
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Synonyms: 84 kDa myeloperoxidase, 89 kDa myeloperoxidase, MPO, MPO Myeloperoxidase, Myeloperoxidase, Myeloperoxidase heavy chain, Myeloperoxidase light chain, PERM_HUMAN
Background: Myeloperoxidase(MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.
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ID gène
- 17523
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NCBI Accession
- NP_034954
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UniProt
- P11247
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Pathways
- Chromatin Binding
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