TIMP2 Kit ELISA

N° du produit ABIN6720136

Détail du produit TIMP2 Kit ELISA

Antigène: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CSC 21K, CSC21K, D11Bwg1104e, DDC8, Metalloproteinase inhibitor 2, Metalloproteinase inhibitor 2 precursor, MGC105282, OTTMUSP00000004170, OTTMUSP00000004171, RP23-394O9.2, TIMP 2, TIMP metallopeptidase inhibitor 2, Tissue inhibitor of metalloproteinase 2, Tissue inhibitor of metalloproteinases 2

Background: TIMP-2 gen is encoded by 5 exons spanning 83 kb of genomic DNA. TIMP-2 is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among the three members of the TIMP family. The gene for tissue inhibitor of metalloproteinases-2 is localized on human chromosome arm 17q25. TIMP-2 abrogates angiogenic factor-induced endothelial cell proliferation in vitro and angiogenesis in vivo independent of MMP inhibition. The standard product used in this kit is recombinant human TIMP-2 with the molecular mass of 22Kda and 194 amino acid.

Gene Name: TIMP2

Production: Natural and recombinant mouse TIMP-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: C27-P220

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse TIMP-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TIMP-2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Autre désignation: TIMP-2 (TIMP2 Produits)

Synonymes: CSC-21K Kit ELISA, DDC8 Kit ELISA, timp2l Kit ELISA, zgc:65967 Kit ELISA, zgc:85799 Kit ELISA, D11Bwg1104e Kit ELISA, Timp-2 Kit ELISA, TIMP-2 Kit ELISA, etID32613.12 Kit ELISA, fa96h09 Kit ELISA, timp2 Kit ELISA, wu:fa96h09 Kit ELISA, zgc:136726 Kit ELISA, TIMP metallopeptidase inhibitor 2 Kit ELISA, TIMP metallopeptidase inhibitor 2b Kit ELISA, tissue inhibitor of metalloproteinase 2 Kit ELISA, TIMP metallopeptidase inhibitor 2a Kit ELISA, TIMP metallopeptidase inhibitor 2 L homeolog Kit ELISA, TIMP2 Kit ELISA, timp2b Kit ELISA, Timp2 Kit ELISA, timp2a Kit ELISA, timp2.L Kit ELISA

ID gène: 21858

NCBI Accession: NP_035724

UniProt: P25785

Pathways: cAMP Metabolic Process