Endothelin Kit ELISA

N° du produit ABIN6720146

N° du produit (Fournisseur): koa0582

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat Endothelin.

Aperçu rapide pour Endothelin Kit ELISA (ABIN6720146)

Antigène: Endothelin

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.9 pg/mL - 250 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit Endothelin Kit ELISA

Seuil minimal de détection: 3.9 pg/mL

N° du produit (Fournisseur): koa0582

Fournisseur: Rockland

Fonction: Rat Endothelin Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant rat Endothelin. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 0.5 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 16.5pg/mL, 7.8pg/mL, 3.9pg/mL rat Endothelin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each rat Endothelin standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: , Immunogen sequence: C53-W73

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Endothelin

Sujet:

Synonyms: Big endothelin 1, ET1, HDLCQ7, PPET1, Preproendothelin 1, EDN2, EDN2_HUMAN, Endothelin 2, Endothelin-2, ET 2, ET-2, ET2, PPET2, Preproendothelin 2, Preproendothelin-2, EDN3, EDN3_HUMAN, Endothelin-3, ET 3, ET-3, PPET3, Preproendothelin 3, Preproendothelin-3

Background: The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure(hypertension) and heart disease.

ID gène: 24323

NCBI Accession: NP_036680

UniProt: P22388