CCL17 Kit ELISA

N° du produit ABIN6720150

N° du produit (Fournisseur): koa0394

Le kit ELISA CCL17 (ABIN6720150) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain CCL17.

Aperçu rapide pour CCL17 Kit ELISA (ABIN6720150)

Antigène: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit CCL17 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

N° du produit (Fournisseur): koa0394

Fournisseur: Rockland

Fonction: Human CCL17 - TARC Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: A24-S94

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 1 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human CCL17 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL17 standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 4.2%, 4.3%, 5.7%
Inter-Assay Precision (CV%): 4.8%, 5.2%, 6.7%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CCL17 (Chemokine (C-C Motif) Ligand 17 (CCL17))

Autre désignation: CCL17/TARC

Sujet:

Background: Chemokine (C-C motif) ligand 17 (CCL17) is a small cytokine belonging to the CC chemokine family that is also known as thymus and activation regulated chemokine (TARC). CCL17 is expressed constitutively in thymus, but only transiently in phytohemagglutinin-stimulated peripheral blood mononuclear cells.1 This chemokine specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4.1,2The gene for CCL17 is located on chromosome 16, in humans, along with other chemokines called CCL22 and CX3CL1.3,4 The standard used in this kit is recombinant human CCL17, consisting of 71 amino acids with the molecular weight of 8Kda.

Gene Full Name: C-C motif chemokine ligand 17

ID gène: 6361

NCBI Accession: NP_002978

UniProt: Q92583