PI3 Kit ELISA

N° du produit ABIN6720164

Détail du produit PI3 Kit ELISA

Antigène: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Cementoin, Elafin, Elastase specific inhibitor, ESI, MGC13613, Peptidase inhibitor 3, Peptidase inhibitor 3 skin derived, PI 3, PI3, Pre elafin, Protease inhibitor WAP3, SKALP, Skin derived antileukoproteinase, Trappin 2, WAP four disulfide core domain 14, WAP four disulfide core domain protein 14, WAP3, WFDC14

Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase(SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core(WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Gene Name: PI3

Production: Natural and recombinant human Elafin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A23-Q117

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Elafin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human Elafin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Autre désignation: Elafin - PI3 (PI3 Produits)

Synonymes: ESI Kit ELISA, SKALP Kit ELISA, WAP3 Kit ELISA, WFDC14 Kit ELISA, cementoin Kit ELISA, trappin-2 Kit ELISA, TRAPPIN-2 Kit ELISA, elafin Kit ELISA, peptidase inhibitor 3 Kit ELISA, peptidase inhibitor 3, skin-derived (SKALP) Kit ELISA, PI3 Kit ELISA

ID gène: 5266

NCBI Accession: NP_002629

UniProt: P19957