ESM1 Kit ELISA

N° du produit ABIN6720165

N° du produit (Fournisseur): koa0444

Ce kit ELISA Humain ESM1 est un kit ELISA Colorimetric conçu pour quantifier Humain ESM1.

Aperçu rapide pour ESM1 Kit ELISA (ABIN6720165)

Antigène: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit ESM1 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

N° du produit (Fournisseur): koa0444

Fournisseur: Rockland

Fonction: Human ESM1 - Endocan Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human ESM1. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human ESM1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ESM1 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: W20-R184

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: ESM1 (Endothelial Cell-Specific Molecule 1 (ESM1))

Autre désignation: ESM1/Endocan

Sujet:

Synonyms: Endothelial cell-specific molecule 1, ESM-1, Esm1, ESM1_HUMAN

Background: Endothelial cell-specific molecule 1, also known as Endocan, is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. ESM1 has been described as a specific biomarker of tip cells during neoangiogenesis by independent teams. Its expression has been shown to be increase in presence of pro-angiogenic growth factors such as VEGF(vascular endothelial growth factor) or FGF-2 (fibroblast growth factor 2).

ID gène: 11082

NCBI Accession: NP_001129076

UniProt: Q9NQ30

Pathways: Growth Factor Binding