IL16 Kit ELISA

N° du produit ABIN6720170

Détail du produit IL16 Kit ELISA

Antigène: IL16 (Interleukin 16 (IL16))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: FLJ16806, FLJ42735, FLJ44234, HsT19289, IL-16, IL16, IL16_HUMAN, Interleukin 16, Interleukin 16 precursor, Interleukin-16, KIAA4048, LCF, Lymphocyte chemoattractant factor, mKIAA4048, Neuronal interleukin 16, NIL16, PrIL16, Pro interleukin 16, Prointerleukin 16

Background: Interleukin 16(IL-16) is a cytokine that released by a variety of cells(including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor(LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.

Gene Name: IL16

Production: Natural and recombinant human IL-16. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence:  P2-S130

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-16 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-16 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: IL16 (Interleukin 16 (IL16))

Autre désignation: IL-16 (IL16 Produits)

Synonymes: IL16 Kit ELISA, LCF Kit ELISA, NIL16 Kit ELISA, PRIL16 Kit ELISA, prIL-16 Kit ELISA, mKIAA4048 Kit ELISA, IL-16 Kit ELISA, interleukin 16 Kit ELISA, IL16 Kit ELISA, Il16 Kit ELISA

ID gène: 3603

NCBI Accession: NP_001165599

UniProt: Q14005