IL-17 Kit ELISA

N° du produit ABIN6720171

Détail du produit IL-17 Kit ELISA

Antigène: IL-17 (IL17) (Interleukin 17 (IL17))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CTLA 8, CTLA-8, CTLA8, Cytotoxic T lymphocyte associated antigen 8, Cytotoxic T lymphocyte associated protein 8, Cytotoxic T lymphocyte associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL 17, IL 17A, IL-17, IL-17A, IL17_HUMAN, Il17a, Interleukin 17A, Interleukin-17A, Interleukin17, Interleukin17A

Background: IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57 % identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri(HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.

Gene Name: IL17A

Production: Natural and recombinant human IL-17. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence:  I20-A155

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-17 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum and plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-17 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: IL-17 (IL17) (Interleukin 17 (IL17))

Autre désignation: IL-17 (IL17 Produits)

Synonymes: CTLA8 Kit ELISA, IL-17 Kit ELISA, IL-17A Kit ELISA, IL17 Kit ELISA, Ctla-8 Kit ELISA, Ctla8 Kit ELISA, Il17 Kit ELISA, ChIL-17 Kit ELISA, IL-17F Kit ELISA, IL17A Kit ELISA, CTLA-8 Kit ELISA, interleukin 17A Kit ELISA, IL17A Kit ELISA, Il17a Kit ELISA

ID gène: 3605

NCBI Accession: NP_002181

UniProt: Q16552