CCL21 Kit ELISA

N° du produit ABIN6720217

N° du produit (Fournisseur): koa0335

Kit ELISA Humain CCL21, test Colorimetric pour la quantification de Humain CCL21.

Aperçu rapide pour CCL21 Kit ELISA (ABIN6720217)

Antigène: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit CCL21 Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

N° du produit (Fournisseur): koa0335

Fournisseur: Rockland

Fonction: Human CCL21 - 6Ckine Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human CCL21. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 12 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human CCL21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CCL21 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: S24-P134

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Autre désignation: CCL21/6Ckine

Sujet:

Synonyms: 6Ckine, Beta chemokine exodus 2, C-C motif chemokine 21, Chemokine (C-C motif) ligand 21, CKb9, ECL, Efficient Chemoattractant for Lymphocytes, Exodus2, MGC34555, OTTHUMP00000000526, OTTHUMP00000000527, OTTHUMP00000021303, SCYA 21, SCYA21, Secondary lymphoid tissue chemokine, SLC, Small inducible cytokine A21, Small inducible cytokine subfamily A (Cys-Cys), member 21, TCA 4, TCA4

Background: Chemokine(C-C motif) ligand 21(CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine(because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine(SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13(CXCL13), CC chemokine ligand 21(CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

ID gène: 6366

NCBI Accession: NP_002980

UniProt: O00585

Pathways: Regulation of Actin Filament Polymerization