Chitinase 3-Like 1/YKL-40 Kit ELISA

N° du produit ABIN6720219

Détail du produit

Antigène: Chitinase 3-Like 1/YKL-40

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: 39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP-39, CGP39, CH3L1_HUMAN, CHI3L1, chitinase 3 like 1 (cartilage glycoprotein 39), chitinase 3 like 1, Chitinase 3 like protein 1 precursor, chitinase, Chitinase-3-like protein 1, GP 39, GP-39, GP39, HC gp39, HCGP 3P, hCGP-39, HCgp39, YKL 40, YKL-40, YKL40, YYL 40

Background: Chitinase-3-like protein 1, also called CHI3L1 or YKL-40 is a secreted glycoprotein that is approximately 40 kDa in size that in humans is encoded by the CHI3L1 gene. This gene is mapped to 1q32.1. Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.

Gene Name: CHI3L1

Production: Natural and recombinant human Chitinase 3-like 1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Y22-T383

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human Chitinase 3-like 1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. We recommend that each human Chitinase 3-like 1 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Other Performance Data: DOE: 2-8° C - 8/23/18 -20° C - 2/23/19

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Chitinase 3-Like 1/YKL-40

Autre désignation: Chitinase 3-like 1 - YKL-40

Synonymes: ASRT7 Kit ELISA, CGP-39 Kit ELISA, GP-39 Kit ELISA, GP39 Kit ELISA, HC-gp39 Kit ELISA, HCGP-3P Kit ELISA, YKL-40 Kit ELISA, YKL40 Kit ELISA, YYL-40 Kit ELISA, hCGP-39 Kit ELISA, chitinase 3 like 1 Kit ELISA, CHI3L1 Kit ELISA

ID gène: 1116

NCBI Accession: NP_001267

UniProt: P36222