ANGPTL4 Kit ELISA

N° du produit ABIN6720260

N° du produit (Fournisseur): koa0590

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain ANGPTL4.

Aperçu rapide pour ANGPTL4 Kit ELISA (ABIN6720260)

Antigène: ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625 pg/mL - 40000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit ANGPTL4 Kit ELISA

Seuil minimal de détection: 625 pg/mL

N° du produit (Fournisseur): koa0590

Fournisseur: Rockland

Fonction: Human ANGPTL4 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: L165-S406

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 20 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 40000pg/mL, 20000pg/mL, 10000pg/mL, 5000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human ANGPTL4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ANGPTL4 standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 6.6%, 5.6%, 4.5%
Inter-Assay Precision (CV%): 7.1%, 6.7%, 4.6%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: ANGPTL4 (Angiopoietin-Like 4 (ANGPTL4))

Autre désignation: ANGPTL4

Sujet:

Background: Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Gene Full Name: angiopoietin like 4

ID gène: 51129

NCBI Accession: NP_001034756

UniProt: Q9BY76

Pathways: Regulation of Lipid Metabolism by PPARalpha