Trefoil Factor 2 Kit ELISA

N° du produit ABIN6720262

N° du produit (Fournisseur): koa0725

Kit ELISA Trefoil Factor 2 Humain, Colorimetric test pour la quantification de Humain Trefoil Factor 2.

Aperçu rapide pour Trefoil Factor 2 Kit ELISA (ABIN6720262)

Antigène: Trefoil Factor 2 (TFF2)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit Trefoil Factor 2 Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

N° du produit (Fournisseur): koa0725

Fournisseur: Rockland

Fonction: Human TFF2 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: E24-Y129

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human TFF2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TFF2 standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 5.3%, 6.6%, 5.5%
Inter-Assay Precision (CV%): 6.2%, 7.4%, 5.8%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Trefoil Factor 2 (TFF2)

Autre désignation: TFF2

Sujet:

Background: Trefoil factor 2, also known as SP or SML1, is a protein that in humans is encoded by the TFF2 gene. This gene belongs to trefoil family, and members of the trefoil family may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene is mapped to 21q22.3. TFF2 gene consists of 4 exons spanning 5.1 kb, with exons 2 and 3 encoding 1 trefoil domain each, and it is the only gene encoding 2 TFF-domains on separate exons. It has been found that the distribution of TFF2 and pS2 suggests involvement in repair-enhancing mechanisms.

Gene Full Name: trefoil factor 2

ID gène: 7032

NCBI Accession: NP_005414

UniProt: Q03403