Trefoil Factor 2 Kit ELISA

N° du produit ABIN6720262

Détail du produit Trefoil Factor 2 Kit ELISA

Antigène: Trefoil Factor 2 (TFF2)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8 pg/mL - 500 pg/mL

Seuil minimal de détection: 7.8 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: SML 1, SML1, SP, Spasmolysin, Spasmolytic polypeptide, spasmolytic protein 1, TFF 2, TFF2, TFF2_HUMAN, trefoil factor 2 (spasmolytic protein 1), Trefoil factor 2, Trefoil factor 2 precursor

Background: Trefoil factor 2, also known as SP or SML1, is a protein that in humans is encoded by the TFF2 gene. This gene belongs to trefoil family, and members of the trefoil family may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. This gene is mapped to 21q22.3. TFF2 gene consists of 4 exons spanning 5.1 kb, with exons 2 and 3 encoding 1 trefoil domain each, and it is the only gene encoding 2 TFF-domains on separate exons. It has been found that the distribution of TFF2 and pS2 suggests involvement in repair-enhancing mechanisms.

Gene Name: TFF2

Production: Natural and recombinant human TFF2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: E24-Y129

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human TFF2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TFF2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 7.8pg/mL-500pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Trefoil Factor 2 (TFF2)

Autre désignation: TFF2 (TFF2 Produits)

Synonymes: TFF2 Kit ELISA, SML1 Kit ELISA, SP Kit ELISA, PSP Kit ELISA, p4 Kit ELISA, p4-a Kit ELISA, sml1 Kit ELISA, xP4 Kit ELISA, xp4.1 Kit ELISA, mSP Kit ELISA, trefoil factor 2 Kit ELISA, trefoil factor 2 S homeolog Kit ELISA, trefoil factor 2 (spasmolytic protein 1) Kit ELISA, TFF2 Kit ELISA, Tff2 Kit ELISA, tff2.S Kit ELISA

ID gène: 7032

NCBI Accession: NP_005414

UniProt: Q03403