REG4 Kit ELISA

N° du produit ABIN6720301

Détail du produit REG4 Kit ELISA

Antigène: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Gastrointestinal secretory protein, GISP, REG IV, REG like protein, REG-4, REG-like protein, Reg4, REG4_HUMAN, Regenerating gene type IV, Regenerating islet derived family member 4, Regenerating islet derived protein 4 precursor, Regenerating islet-derived protein 4, Regenerating islet-derived protein IV, REGIV, RELP

Background: Regenerating islet-derived protein 4 is a protein that in humans is encoded by the REG4 gene. It is mapped to 1p12. REG-4 gene contains 6 exons and that the exon structure is preserved among members of the REG gene family. This gene is able to maintain carbohydrate recognition activity in an acidic environment. REG-4 expression was significantly upregulated by inflammation and tissue injury associated with active Crohn disease and ulcerative colitis, and most of the colorectal tumors overexpressing REG-4 are mucinous tumors or neuroendocrine tumors.

Gene Name: REG4

Production: Natural and recombinant human REG-4. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: D23-P158

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human REG-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each human REG-4 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: REG4 (Regenerating Islet-Derived Family, Member 4 (REG4))

Autre désignation: REG-4 (REG4 Produits)

Synonymes: GISP Kit ELISA, REG-IV Kit ELISA, RELP Kit ELISA, 2010002L15Rik Kit ELISA, regenerating family member 4 Kit ELISA, regenerating islet-derived family, member 4 Kit ELISA, Reg4 Kit ELISA, REG4 Kit ELISA

ID gène: 83998

NCBI Accession: NP_001152824

UniProt: Q9BYZ8