PTX3 Kit ELISA

N° du produit ABIN6720329

N° du produit (Fournisseur): koa0521

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain PTX3.

Aperçu rapide pour PTX3 Kit ELISA (ABIN6720329)

Antigène: PTX3 (Pentraxin 3 (PTX3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Saliva, Serum

Détail du produit PTX3 Kit ELISA

Seuil minimal de détection: 312 pg/mL

N° du produit (Fournisseur): koa0521

Fournisseur: Rockland

Fonction: Human PTX3 - Pentraxin 3 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: E18-S381

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human PTX3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(EDTA) or saliva to each empty well. It is recommended that each human PTX3 standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 6.9%, 5%, 6.9%
Inter-Assay Precision (CV%): 5.4%, 6.9%, 6.9%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: PTX3 (Pentraxin 3 (PTX3))

Autre désignation: PTX3/Pentraxin 3

Sujet:

Background: PTX3 (Pentraxin 3) is a member of the pentraxin superfamily. This super family characterized by cyclic multimeric structure. The PTX3 gene is mapped to 3q25.32. The predicted 381-amino acid PTX3 protein has homology to the pentraxin protein family. Significant levels of PTX3 were detected in plasma of neutropenic patients with systemic A. PTX3 is effective in preventing CMV infection and reactivation, as well as subsequent Aspergillus infection. PTX3 activates the classical pathway of complementactivation and facilitates pathogen recognition by macrophages and DCs.

Gene Full Name: pentraxin 3

ID gène: 5806

NCBI Accession: NP_002843

UniProt: P26022