TIMP4 Kit ELISA

N° du produit ABIN6720333

N° du produit (Fournisseur): koa0316

Le kit ELISA TIMP4 (ABIN6720333) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain TIMP4.

Aperçu rapide pour TIMP4 Kit ELISA (ABIN6720333)

Antigène: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit TIMP4 Kit ELISA

Seuil minimal de détection: 78 pg/mL

N° du produit (Fournisseur): koa0316

Fournisseur: Rockland

Fonction: Human TIMP-4 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: C30-P224

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human TIMP-4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin , EDTA) or human milk to each empty well. We recommend that each human TIMP-4 standard solution and each sample is measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 7.7%, 6.6%, 6.1%
Inter-Assay Precision (CV%): 9%, 8%, 7.9%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: TIMP4 (TIMP Metallopeptidase Inhibitor 4 (TIMP4))

Autre désignation: TIMP-4

Sujet:

Background: Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

Gene Full Name: TIMP metallopeptidase inhibitor 4

ID gène: 7079

NCBI Accession: NP_003247

UniProt: Q99727