AGRP Kit ELISA

N° du produit ABIN6720335

N° du produit (Fournisseur): koa0862

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain AGRP.

Aperçu rapide pour AGRP Kit ELISA (ABIN6720335)

Antigène: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8 pg/mL - 500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine

Détail du produit AGRP Kit ELISA

Seuil minimal de détection: 7.8 pg/mL

N° du produit (Fournisseur): koa0862

Fournisseur: Rockland

Fonction: Human AGRP - agouti-related Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: A21-T132

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 4 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL, 7.8pg/mL human AGRP standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human AGRP standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 4.2%, 4%, 4.4%
Inter-Assay Precision (CV%): 5.3%, 5.3%, 4.9%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: AGRP (Agouti Related Protein Homolog (Mouse) (AGRP))

Autre désignation: AGRP

Sujet:

Background: Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

Gene Full Name: agouti related neuropeptide

ID gène: 181

NCBI Accession: NP_001129

UniProt: O00253

Pathways: Feeding Behaviour, Photoperiodism