MIA Kit ELISA

N° du produit ABIN6720344

Détail du produit MIA Kit ELISA

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD RAP, melanoma inhibitory activity, Melanoma inhibitory activity protein, Melanoma-derived growth regulatory protein, Mia, MIA_HUMAN

Background: Melanoma Inhibiting Activity(MIA), also known as cartilage-derived retinoic acid-sensitive protein(CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain(1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .

Gene Name: MIA

Production: Natural and recombinant human MIA. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: G25-131Q

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or saliva to each empty well. It is recommended that each human MIA standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: MIA (Melanoma Inhibitory Activity (MIA))

Autre désignation: MIA (MIA Produits)

Synonymes: CD-RAP Kit ELISA, Mia1 Kit ELISA, Cdrap Kit ELISA, zgc:194157 Kit ELISA, mia Kit ELISA, CDRAP Kit ELISA, Fdp Kit ELISA, MIA Kit ELISA, MIAL Kit ELISA, melanoma inhibitory activity Kit ELISA, otoraplin Kit ELISA, cilia and flagella associated protein 100 Kit ELISA, MIA Kit ELISA, Mia Kit ELISA, mia Kit ELISA, Otor Kit ELISA, CFAP100 Kit ELISA

ID gène: 8190

NCBI Accession: NP_001189482

UniProt: Q16674