PRSS8 Kit ELISA

N° du produit ABIN6720348

N° du produit (Fournisseur): koa0749

Ce kit ELISA Souris PRSS8 est un kit ELISA Colorimetric conçu pour quantifier Souris PRSS8.

Aperçu rapide pour PRSS8 Kit ELISA (ABIN6720348)

Antigène: PRSS8 (Protease, serine, 8 (PRSS8))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Détail du produit PRSS8 Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

N° du produit (Fournisseur): koa0749

Fournisseur: Rockland

Fonction: Mouse Prostasin Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant mouse Prostasin. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 50 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL mouse Prostasin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. We recommend that each mouse Prostasin standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: A30-Q289

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: PRSS8 (Protease, serine, 8 (PRSS8))

Autre désignation: Prostasin

Sujet:

Synonyms: 2410039E18Rik, AI313909, C79772, CAP1, Channel Activating Protease 1, mCAP1, Prostasin, Prostasin heavy chain, protease, serine, 8, Prss8, PRSS8_HUMAN, Serine protease 9

Background: Prostasin is a protein that in humans is encoded by the PRSS8 gene. It is a single-copy gene and mapped to 16p11.2. Prostasin is likely to be the more physiologically relevant protease. This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. Coexpression of Prostasin with either Xenopus or rat ENaC in Xenopus oocytes resulted in a 60 % to 80 % increase in amiloride-sensitive sodium currents, and that the addition of aprotinin, a serine protease inhibitor, completely prevented this activation. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine.

ID gène: 76560

NCBI Accession: NP_579929

UniProt: Q9ESD1