ADIPOQ Kit ELISA

N° du produit ABIN6720368

N° du produit (Fournisseur): koa0365

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain ADIPOQ.

Aperçu rapide pour ADIPOQ Kit ELISA (ABIN6720368)

Antigène: ADIPOQ (Adiponectin (ADIPOQ))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56 ng/mL - 100 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Urine

Détail du produit ADIPOQ Kit ELISA

Seuil minimal de détection: 1.56 ng/mL

N° du produit (Fournisseur): koa0365

Fournisseur: Rockland

Fonction: Human Adiponectin Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: E19-N244

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 60 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. It is recommended that each human Adiponectin standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 4.9%, 7.8%, 4.7%
Inter-Assay Precision (CV%): 6.5%, 9%, 5.2%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: ADIPOQ (Adiponectin (ADIPOQ))

Autre désignation: Adiponectin

Sujet:

Background: Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.

Gene Full Name: adiponectin, C1Q and collagen domain containing

ID gène: 9370

NCBI Accession: NP_001171271

UniProt: Q15848