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ADAM12 Kit ELISA (ADAM Metallopeptidase Domain 12) Kit ELISA

ADAM12 Reactivité: Humain Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant
N° du produit ABIN6720375
$504.00
Plus shipping costs $45.00
1 kit
local_shipping Destination: Etats-Unis
Envoi sous 1 à 2 jours ouvrables
  • Antigène
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156 pg/mL - 10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich ELISA for Quantitative Detection of Antigen
    Type d'échantillon
    Cell Culture Supernatant, Plasma (heparin), Serum, Urine
    Analytical Method
    Quantitative
    Attributs du produit

    Synonyms: ADA12_HUMAN, ADAM 12, ADAM metallopeptidase domain 12, ADAM12, Disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, Meltrin-alpha, MLTN, MLTNA

    Background: ADAM12(Disintegrin and metalloproteinase domain-containing protein 12), also known as MLTN, is an enzyme that in humans is encoded by the ADAM12 gene. This gene encodes a member of the ADAM(a disintegrin and metalloprotease) protein family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This gene has two alternatively spliced transcripts: a shorter secreted form and a longer membrane-bound form. The shorter form is found to stimulate myogenesis. By RT-PCR and immunoblot analyses that expression of mouse Adam12 increases during muscle regeneration, while the levels of other ADAMs remain constant. Immunofluorescence analysis revealed staining of small, newly formed muscle fibers in regenerating but not normal adult muscle cells. By using of fluorescence in situ hybridization, the ADAM12 gene is mapped to human chromosome 10q26.3.

    Gene Name: ADAM12

    Production: Natural and recombinant human ADAM12. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: CHO, Immunogen sequence: R29-S513

  • Indications d'application

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human ADAM12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin) or urine to each empty well. We recommend that each human ADAM12 standard solution and each sample is measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 156pg/mL-10000pg/mL

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Antigène
    Autre désignation
    ADAM12 (ADAM12 ELISA Kit Extrait)
    ID gène
    8038
    NCBI Accession
    NP_001275903
    UniProt
    O43184
    Pathways
    EGFR Signaling Pathway
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