Lipocalin 2 Kit ELISA

N° du produit ABIN6720376

N° du produit (Fournisseur): koa0515

Kit ELISA Souris Lipocalin 2, test Colorimetric pour la quantification de Souris Lipocalin 2.

Aperçu rapide pour Lipocalin 2 Kit ELISA (ABIN6720376)

Antigène: Lipocalin 2 (LCN2)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (heparin), Serum, Urine

Détail du produit Lipocalin 2 Kit ELISA

Seuil minimal de détection: 78 pg/mL

N° du produit (Fournisseur): koa0515

Fournisseur: Rockland

Fonction: Mouse Lipocalin-2 - NGAL Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant mouse NGAL. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse NGAL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin) or urine to each empty well. It is recommended that each mouse NGAL standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: Q21-N200

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Lipocalin 2 (LCN2)

Autre désignation: Lipocalin-2/NGAL

Sujet:

Synonyms: 24p3, 25 kDa alpha-2-microglobulin-related subunit of MMP-9, HNL, Lcn2, Lipocalin 2, Lipocalin-2, Migration stimulating factor inhibitor, MSFI, Neutrophil gelatinase-associated lipocalin, NGAL, NGAL_HUMAN, Oncogene 24p3, p25, Siderocalin, Siderocalin LCN3

Background: Lipocalin-2(LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide(LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant mouse NGAL, consisting of 179 amino acids with the molecular mass of 22KDa.

ID gène: 16819

NCBI Accession: NP_032517

UniProt: P11672

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis