Osteopontin Kit ELISA
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- Antigène Voir toutes Osteopontin (SPP1) Kits ELISA
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 156 pg/mL - 10000 pg/mL
- Seuil minimal de détection
- 156 pg/mL
- Application
- ELISA
- Fonction
- Sandwich ELISA for Quantitative Detection of Antigen
- Type d'échantillon
- Cell Culture Supernatant, Plasma (heparin), Saliva, Serum, Urine
- Analytical Method
- Quantitative
- Attributs du produit
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Synonyms: BNSP, Bone sialoprotein 1, Bone sialoprotein I, BSPI, Early T lymphocyte activation 1, ETA 1, MGC110940, Nephropontin, OPN, Osteopontin, OSTP_HUMAN, Secreted phosphoprotein 1, Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1), Secreted phosphoprotein 1 (osteopontin bone sialoprotein), SPP-1, SPP1, Urinary stone protein, Uropontin
Background: Osteopontin(OPN) also called urinary stone protein, secreted phosphoprotein 1(SPP1), bone sialoprotein, and early T lymphocyte activation 1(ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein(34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant human OPN, consisting of 17-314 amino acid sequence with the molecular mass of 32.9KDa. As a result of glycosylation, the molecular mass is 60-65KDa.
Gene Name: SPP1
Production: Natural and recombinant human OPN. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: NSO, Immunogen sequence: E22-L401
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- Discover our top product SPP1 Kit ELISA
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- Indications d'application
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human OPN standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin), saliva or urine to each empty well. It is recommended that each human OPN standard solution and each sample be measured in duplicate.
Blood Product Anticoagulant: Heparin Sodium
ELISA Dilution: 156pg/mL-10000pg/mL
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Antigène Voir toutes Osteopontin (SPP1) Kits ELISA
- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
- Autre désignation
- OPN (SPP1 Produits)
- Synonymes
- BNSP Kit ELISA, BSPI Kit ELISA, ETA-1 Kit ELISA, OPN Kit ELISA, 2AR Kit ELISA, Apl-1 Kit ELISA, Bsp Kit ELISA, Eta Kit ELISA, OP Kit ELISA, Opn Kit ELISA, Opnl Kit ELISA, Ric Kit ELISA, Spp-1 Kit ELISA, OSP Kit ELISA, zgc:111821 Kit ELISA, secreted phosphoprotein 1 Kit ELISA, SPP1 Kit ELISA, Spp1 Kit ELISA, spp1 Kit ELISA
- ID gène
- 6696
- NCBI Accession
- NP_000573
- UniProt
- P10451
- Pathways
- Regulation of Cell Size
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