PGD Kit ELISA

N° du produit ABIN6730055

Détail du produit PGD Kit ELISA

Antigène: PGD (Phosphogluconate Dehydrogenase (PGD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human PGD ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human PGD.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: PGD (Phosphogluconate Dehydrogenase (PGD))

Autre désignation: PGD (PGD Produits)

Synonymes: 6PGD Kit ELISA, 6-PGD Kit ELISA, 6-Pgd Kit ELISA, 6-pgd Kit ELISA, 6PGDH Kit ELISA, 6Pgd Kit ELISA, 6Pgdh Kit ELISA, 6pgdh Kit ELISA, A7 Kit ELISA, CG3724 Kit ELISA, Dmel\\CG3724 Kit ELISA, EG:87B1.4 Kit ELISA, N1 Kit ELISA, PGD Kit ELISA, Pdg Kit ELISA, l(1)2Dc Kit ELISA, l(1)2De Kit ELISA, l(1)A7 Kit ELISA, l(1)G0385 Kit ELISA, l(1)N1 Kit ELISA, l(1)N3[90] Kit ELISA, l(1)Pgd Kit ELISA, l(1)Pgd-A Kit ELISA, l35 Kit ELISA, pgd Kit ELISA, 0610042A05Rik Kit ELISA, AU019875 Kit ELISA, C78335 Kit ELISA, fi03d03 Kit ELISA, zgc:55585 Kit ELISA, wu:fi03d03 Kit ELISA, zgc:111848 Kit ELISA, 6-PGDH Kit ELISA, GND1b Kit ELISA, Pgd Kit ELISA, Cc2-27 Kit ELISA, phosphogluconate dehydrogenase Kit ELISA, Phosphogluconate dehydrogenase Kit ELISA, phosphogluconate dehydrogenase L homeolog Kit ELISA, 6-phosphogluconate dehydrogenase, decarboxylating Kit ELISA, 6-phosphogluconate dehydrogenase, decarboxylating-like Kit ELISA, PGD Kit ELISA, Pgd Kit ELISA, pgd.L Kit ELISA, pgd Kit ELISA, GND1a|GND1b Kit ELISA, Thal_1580 Kit ELISA, GY4MC1_3101 Kit ELISA, Odosp_2189 Kit ELISA, Marky_1024 Kit ELISA, gnd Kit ELISA, LOC101451876 Kit ELISA

UniProt: P52209