JAML Kit ELISA

N° du produit ABIN6730068

Kit ELISA JAML Souris, Colorimetric test pour la quantification de Souris JAML.

Aperçu rapide pour JAML Kit ELISA (ABIN6730068)

Antigène: JAML (AMICA1) (Adhesion Molecule, Interacts with CXADR Antigen 1 (AMICA1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.45 ng/mL - 150 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit JAML Kit ELISA

Seuil minimal de détection: 0.45 ng/mL

Fonction: Mouse AMICA (JAML ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse APLP-1, Arylsulfatase G, B7-H5, CD47, CES2, CLEC4F, CPA1, CPB1, CREG, CRELD2, Dectin-1, EMMPRIN, FABP4, FCRL5, FGF R5, FOLR1, Galectin-2, Glypican 5, ICOS, IDS, IGFBP-1, IL-12 R beta 1, IL-30, IL-4 R alpha, Lefty-1, Legumain, LRIG1, Mcpt7, MDL-1, MGL2, MSP R, NCAM-1, Netrin-G2a, Neuroglycan C, Nidogen-2, PDGF R alpha, PILR-alpha, PIR-B, Plexin B2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: JAML (AMICA1) (Adhesion Molecule, Interacts with CXADR Antigen 1 (AMICA1))

Autre désignation: AMICA (JAML

Sujet:

Gene Symbols: Amica1, Gm638, Jaml

Protein Name (Synonyms): Junctional adhesion molecule-like (Dendritic cell-specific protein CREA7) (mCrea7)

ID gène: 270152

UniProt: Q80UL9