C1QBP Kit ELISA

N° du produit ABIN6730090

Détail du produit C1QBP Kit ELISA

Antigène: C1QBP (Complement Component 1, Q Subcomponent Binding Protein (C1QBP))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse C1QBP ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse C1QBP.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: C1QBP (Complement Component 1, Q Subcomponent Binding Protein (C1QBP))

Autre désignation: C1QBP (C1QBP Produits)

Synonymes: SF2 Kit ELISA, X38k Kit ELISA, gC1qR Kit ELISA, C1QBP Kit ELISA, GC1QBP Kit ELISA, HABP1 Kit ELISA, SF2p32 Kit ELISA, gC1Q-R Kit ELISA, p32 Kit ELISA, AA407365 Kit ELISA, AA986492 Kit ELISA, D11Wsu182e Kit ELISA, P32 Kit ELISA, gC1qBP Kit ELISA, Habp1 Kit ELISA, complement C1q binding protein Kit ELISA, complement component 1, q subcomponent binding protein S homeolog Kit ELISA, complement component 1, q subcomponent binding protein Kit ELISA, C1QBP Kit ELISA, c1qbp.S Kit ELISA, LOC100227158 Kit ELISA, C1qbp Kit ELISA

UniProt: O35658

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly