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CD44 Kit ELISA

CD44 Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730117
  • Antigène Voir toutes CD44 Kits ELISA
    CD44
    Reactivité
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse CD44 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse CD44.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CD44 Kits ELISA
    CD44
    Autre désignation
    CD44 (CD44 Produits)
    Synonymes
    CDW44 Kit ELISA, CSPG8 Kit ELISA, ECMR-III Kit ELISA, HCELL Kit ELISA, HUTCH-I Kit ELISA, IN Kit ELISA, LHR Kit ELISA, MC56 Kit ELISA, MDU2 Kit ELISA, MDU3 Kit ELISA, MIC4 Kit ELISA, Pgp1 Kit ELISA, AU023126 Kit ELISA, AW121933 Kit ELISA, AW146109 Kit ELISA, HERMES Kit ELISA, Ly-24 Kit ELISA, Pgp-1 Kit ELISA, CD44A Kit ELISA, METAA Kit ELISA, RHAMM Kit ELISA, XCD44 Kit ELISA, cd44 Kit ELISA, cdw44 Kit ELISA, cspg8 Kit ELISA, ecmr-iii Kit ELISA, hcell Kit ELISA, lhr Kit ELISA, mc56 Kit ELISA, mdu2 Kit ELISA, mdu3 Kit ELISA, mic4 Kit ELISA, mutch-i Kit ELISA, pgp1 Kit ELISA, CD44 molecule (Indian blood group) Kit ELISA, syndecan 4 Kit ELISA, CD44 antigen Kit ELISA, CD44 molecule (Indian blood group) S homeolog Kit ELISA, CD44 Kit ELISA, SDC4 Kit ELISA, Cd44 Kit ELISA, cd44.S Kit ELISA
    UniProt
    P15379
    Pathways
    Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling
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