Cathepsin L Kit ELISA

N° du produit ABIN6730136

Détail du produit Cathepsin L Kit ELISA

Antigène: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Cathepsin L ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse Cathepsin L.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Autre désignation: Cathepsin L (CTSL1 Produits)

Synonymes: CATL Kit ELISA, CTSL1 Kit ELISA, MEP Kit ELISA, 1190035F06Rik Kit ELISA, Ctsl1 Kit ELISA, fs Kit ELISA, nkt Kit ELISA, CATHL Kit ELISA, CatL Kit ELISA, Ctsl Kit ELISA, CTSL Kit ELISA, mep Kit ELISA, catl Kit ELISA, ctsl Kit ELISA, cpl-1 Kit ELISA, MGC80629 Kit ELISA, MGC107932 Kit ELISA, LOC663234 Kit ELISA, 26-29-p Kit ELISA, Cp1 Kit ELISA, si:dkey-121a11.6 Kit ELISA, CTSV Kit ELISA, catL Kit ELISA, cb15 Kit ELISA, ctsl1b Kit ELISA, hgg1 Kit ELISA, wu:fb30g09 Kit ELISA, zgc:92089 Kit ELISA, cathepsin L Kit ELISA, cathepsin V Kit ELISA, cathepsin L L homeolog Kit ELISA, cathepsin L1 Kit ELISA, Cathepsin L1 Kit ELISA, Cathepsin L Kit ELISA, testin gene Kit ELISA, cathepsin 12 Kit ELISA, cathepsin Lb Kit ELISA, cathepsin L.1 Kit ELISA, CTSL Kit ELISA, Ctsl Kit ELISA, CTSV Kit ELISA, ctsl.L Kit ELISA, ctsl Kit ELISA, LOC100008830 Kit ELISA, catl1 Kit ELISA, 26-29-p Kit ELISA, catl Kit ELISA, Tsp_11596 Kit ELISA, Testin Kit ELISA, cts12 Kit ELISA, LOC102156614 Kit ELISA, ctslb Kit ELISA, ctsl.1 Kit ELISA

UniProt: Q543M3

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades