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Coagulation Factor IX Kit ELISA
F9
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730141
Détail du produit Coagulation Factor IX Kit ELISA
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Antigène
Voir toutes Coagulation Factor IX (F9) Kits ELISA
Coagulation Factor IX (F9)
Reactivité
Toutes les réactivités sur Coagulation Factor IX Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse Coagulation Factor IX/F9 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse Coagulation Factor IX.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternatives
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes Coagulation Factor IX (F9) Kits ELISA
Coagulation Factor IX (F9)
Autre désignation
Coagulation Factor IX (F9 (F9 Produits )
Synonymes
AA407498 Kit ELISA, AI506714 Kit ELISA, AK-3 Kit ELISA, Ak3l Kit ELISA, Ak3l1 Kit ELISA, Akl3l Kit ELISA, FIX Kit ELISA, HEMB Kit ELISA, P19 Kit ELISA, PTC Kit ELISA, THPH8 Kit ELISA, AW111646 Kit ELISA, Cf-9 Kit ELISA, Cf9 Kit ELISA, f9 Kit ELISA, zgc:109775 Kit ELISA, fix Kit ELISA, ptc Kit ELISA, hemb Kit ELISA, FIXA Kit ELISA, zgc:136807 Kit ELISA, adenylate kinase 3 Kit ELISA, coagulation factor IX Kit ELISA, coagulation factor IXa Kit ELISA, coagulation factor 9 Kit ELISA, coagulation factor 9 S homeolog Kit ELISA, Coagulation factor IX Kit ELISA, coagulation factor IXb Kit ELISA, Ak3 Kit ELISA, F9 Kit ELISA, f9a Kit ELISA, f9 Kit ELISA, CpipJ_CPIJ003776 Kit ELISA, CpipJ_CPIJ009129 Kit ELISA, CpipJ_CPIJ013063 Kit ELISA, CpipJ_CPIJ013624 Kit ELISA, Tsp_01441 Kit ELISA, f9.S Kit ELISA, fa9 Kit ELISA, f9b Kit ELISA
UniProt
P16294
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