Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

CYGB Kit ELISA

CYGB Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730307
  • Antigène Voir toutes CYGB Kits ELISA
    CYGB (Cytoglobin (CYGB))
    Reactivité
    • 3
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Cytoglobin/CYGB ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human Cytoglobin.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CYGB Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CYGB Kits ELISA
    CYGB (Cytoglobin (CYGB))
    Autre désignation
    Cytoglobin (CYGB (CYGB Produits)
    Synonymes
    cygb Kit ELISA, wu:fb77d07 Kit ELISA, wu:fb93a03 Kit ELISA, zgc:109806 Kit ELISA, MGC89562 Kit ELISA, CYGB Kit ELISA, cygb-1 Kit ELISA, MGC84306 Kit ELISA, 3110001K20Rik Kit ELISA, HGb Kit ELISA, Staap Kit ELISA, HGB Kit ELISA, STAP Kit ELISA, Stap Kit ELISA, cytoglobin 1 Kit ELISA, cytoglobin Kit ELISA, cytoglobin-1 Kit ELISA, cytoglobin L homeolog Kit ELISA, cygb1 Kit ELISA, cygb Kit ELISA, CYGB Kit ELISA, cygb.L Kit ELISA, Cygb Kit ELISA
    UniProt
    Q8WWM9
Vous êtes ici:
Support technique