Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

JAM2 Kit ELISA

JAM2 Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730316
  • Antigène Voir toutes JAM2 Kits ELISA
    JAM2 (Junctional Adhesion Molecule 2 (JAM2))
    Reactivité
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse JAM-B/VE-JAM ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse JAM-B.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product JAM2 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes JAM2 Kits ELISA
    JAM2 (Junctional Adhesion Molecule 2 (JAM2))
    Autre désignation
    JAM-B (VE-JAM (JAM2 Produits)
    Synonymes
    JAM2 Kit ELISA, cd322 Kit ELISA, jam-b Kit ELISA, jamb Kit ELISA, pro245 Kit ELISA, ve-jam Kit ELISA, vejam Kit ELISA, jam2 Kit ELISA, sb:cb1002 Kit ELISA, im:7155552 Kit ELISA, sc:d0813 Kit ELISA, si:ch211-160b11.5 Kit ELISA, LOC100227766 Kit ELISA, 1110002N23Rik Kit ELISA, 2410030G21Rik Kit ELISA, 2410167M24Rik Kit ELISA, JAM-2 Kit ELISA, JAM-B Kit ELISA, Jcam2 Kit ELISA, VE-JAM Kit ELISA, C21orf43 Kit ELISA, CD322 Kit ELISA, JAMB Kit ELISA, PRO245 Kit ELISA, VEJAM Kit ELISA, junctional adhesion molecule 2 Kit ELISA, junctional adhesion molecule 2a Kit ELISA, junctional adhesion molecule 2b Kit ELISA, junction adhesion molecule 2 Kit ELISA, JAM2 Kit ELISA, jam2 Kit ELISA, jam2a Kit ELISA, jam2b Kit ELISA, Jam2 Kit ELISA
    UniProt
    Q9JI59
Vous êtes ici:
Support technique