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UCHL1 Kit ELISA
UCHL1
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730322
Détail du produit UCHL1 Kit ELISA
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Antigène
Voir toutes UCHL1 Kits ELISA
UCHL1
(Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
Reactivité
Toutes les réactivités sur UCHL1 Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse UCHL1 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse UCHL1.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternatives
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes UCHL1 Kits ELISA
UCHL1
(Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
Autre désignation
UCHL1 (UCHL1 Produits )
Synonymes
PARK5 Kit ELISA, PGP 9.5 Kit ELISA, PGP9.5 Kit ELISA, PGP95 Kit ELISA, Uch-L1 Kit ELISA, UCH-L1 Kit ELISA, UCHL1 Kit ELISA, cb358 Kit ELISA, wu:fc55h08 Kit ELISA, park5 Kit ELISA, pgp9.5 Kit ELISA, uch-l1 Kit ELISA, MGC132191 Kit ELISA, uchl1 Kit ELISA, AW822034 Kit ELISA, C88048 Kit ELISA, R75593 Kit ELISA, UCHL-1 Kit ELISA, gad Kit ELISA, ubiquitin C-terminal hydrolase L1 Kit ELISA, ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) Kit ELISA, ubiquitin C-terminal hydrolase L1 L homeolog Kit ELISA, ubiquitin carboxyl-terminal esterase L1 Kit ELISA, ubiquitin carboxy-terminal hydrolase L1 Kit ELISA, UCHL1 Kit ELISA, uchl1 Kit ELISA, uchl1.L Kit ELISA, LOC100304750 Kit ELISA, Uchl1 Kit ELISA
UniProt
Q9R0P9
Pathways
Feeding Behaviour
Vus récemment
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