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Factor VII Kit ELISA
F7
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730340
Détail du produit Factor VII Kit ELISA
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Antigène
Voir toutes Factor VII (F7) Kits ELISA
Factor VII (F7)
(Coagulation Factor VII (F7))
Reactivité
Toutes les réactivités sur Factor VII Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Mouse Coagulation Factor VII/F7 ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Mouse Coagulation Factor VII.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Optimal working dilution should be determined by the investigator.
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes Factor VII (F7) Kits ELISA
Factor VII (F7)
(Coagulation Factor VII (F7))
Autre désignation
Coagulation Factor VII (F7 (F7 Produits )
Synonymes
F7 Kit ELISA, wu:fb59f04 Kit ELISA, zgc:109870 Kit ELISA, SPCA Kit ELISA, AI114908 Kit ELISA, ATIII Kit ELISA, At-3 Kit ELISA, At3 Kit ELISA, LOC100223776 Kit ELISA, AI132620 Kit ELISA, Cf7 Kit ELISA, FVII Kit ELISA, mfVII Kit ELISA, f7 Kit ELISA, coagulation factor VII Kit ELISA, serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 Kit ELISA, coagulation factor 7 (serum prothrombin conversion accelerator) S homeolog Kit ELISA, F7 Kit ELISA, f7 Kit ELISA, Serpinc1 Kit ELISA, CpipJ_CPIJ009142 Kit ELISA, CpipJ_CPIJ010295 Kit ELISA, CpipJ_CPIJ020127 Kit ELISA, fa7 Kit ELISA, f7.S Kit ELISA
UniProt
P70375
Pathways
Response to Growth Hormone Stimulus , Platelet-derived growth Factor Receptor Signaling
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