KNG1 Kit ELISA
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- Antigène Voir toutes KNG1 Kits ELISA
- KNG1 (Kininogen 1 (KNG1))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Mouse Kininogen-1/Kng1 ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Mouse Kininogen-1.
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product KNG1 Kit ELISA
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes KNG1 Kits ELISA
- KNG1 (Kininogen 1 (KNG1))
- Autre désignation
- Kininogen-1 (Kng1 (KNG1 Produits)
- Synonymes
- BDK Kit ELISA, BK Kit ELISA, KNG Kit ELISA, Kng Kit ELISA, KNG2 Kit ELISA, fb64g01 Kit ELISA, wu:fb64g01 Kit ELISA, zgc:103569 Kit ELISA, kininogen Kit ELISA, bdk Kit ELISA, kng Kit ELISA, KNG1 Kit ELISA, IHRP Kit ELISA, KINKG Kit ELISA, KINKH Kit ELISA, Kng1 Kit ELISA, Kngk Kit ELISA, kininogen 1 Kit ELISA, kininogen 1 L homeolog Kit ELISA, inter-alpha-trypsin inhibitor heavy chain family member 4 Kit ELISA, kininogen 2-like 1 Kit ELISA, KNG1 Kit ELISA, Kng1 Kit ELISA, kng1 Kit ELISA, kng1.L Kit ELISA, ITIH4 Kit ELISA, Kng2l1 Kit ELISA
- UniProt
- O08677
- Pathways
- ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process
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